Recombinant equine IL-1RA protein, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg, 200 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Equine (horse) |
| Accession Number | O18999 |
| Gene Symbols | IL1RA |
| Protein Name / Synonyms | Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, IL1 inhibitor. |
| Expressed Region | His26-Gln177 |
| Expression System | HEK293 cells |
| Tag | C-terminal his-tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 17 kDa. Due to the abundant glycosylation, it migrates as multiple large protein bands (17-40 kDa) in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under denature condition, the protein presented as one single 17 kDa band. See deglycosylation analysis in SDS-PAGE image. |
| Format | Liquid |
| Formulation | Filtered solution in 1x PBS (pH 7.4) with 50% glycerol |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay. |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: protein standard ladder (kDa).
Lane 2: untreated protein under reducing conditions.
Lane 3: treated protein with deglycosylation enzymes under native conditions.
Lane 4: treated protein with deglycosylation enzymes under denature conditions.
Lane 5: deglycosylation mixture only without target proteins.
Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Recombinant equine IL-1RA, as a receptor antagonist of IL-1, can inhibit the biological activity of IL-1b.
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
