Recombinant human YWHAE (Tyrosine 3-monooxygenase/Tryptophan 5-monooxygenase activation protein, Epsilon polypeptide), derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 50 µg, 100 µg, 250 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P62258 |
| Gene Id | 7531 |
| Gene Symbols | YWHAE |
| Protein Name / Synonyms | 14-3-3 protein epsilon, 14-3-3E, Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Epsilon, 14-3-3 Epsilon, MDCR, MDS, Tyrosine 3/Tryptophan 5 -Monooxygenase Activation Protein Epsilon Polypeptide, Mitochondrial Import Stimulation Factor L Subunit, Protein Kinase C Inhibitor Protein-1, Epididymis Luminal Protein 2, KCIP-1, HEL2 |
| Expressed Region | Asp2-Gln255 |
| Expression System | HEK293 cells |
| Tag | N-terminal 6x histidine tag |
| Purity | > 90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant mouse 14-3-3 protein epsilon has a calculated molecular mass of 28 kDa. Due to the abundant glycosylation, it migrates as approximately 28-30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
| Endotoxin Level | Not determined |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa).
Lane 2: Untreated protein under reducing conditions.
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Stability & Storage
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
