Biotin-conjugated recombinant human ANXA2 (Annexin A2), derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | P07355 |
| Gene Id | 302 |
| Gene Symbols | ANXA2 |
| Protein Name / Synonyms | Annexin A2, LIP2, ANX2L4, HEL-S-270, LPC2D, LPC2, ANX2, CAL1H, annexin-2, Annexin II, Calpactin I Heavy Polypeptide, Calpactin I Heavy Chain2, Chromobindin 8, Calpactin-1 Heavy Chain2, chromobindin-8, Lipocortin II, Epididymis Secretory Protein Li 270, Placental Anticoagulant Protein IV, Annexin-2, Protein I, Chromobindin-8, PAP-IV, p36, Calpactin-1 heavy chain |
| Expressed Region | Ser2-Asp339 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | N-terminal 6x histidine tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant human ANXA2 has a calculated molecular mass of 40 kDa. Due to the abundant glycosylation, it migrates as approximately 40-45 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
| Endotoxin Level | Not determined |
| Research Area | Angiogenesis, Cancer |
| Protein Type | Recombinant Protein |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa).
Lane 2: Untreated protein under reducing conditions.
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Activity
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
