Recombinant human basigin protein, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 50 µg, 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P35613-2 |
| Gene Symbols | BSG |
| Protein Name / Synonyms | CD147, 5F7, Collagenase stimulatory factor, EMMPRIN, HAb18G, OK blood group antigen, TCSF |
| Expressed Region | Thr28-Leu206 |
| Expression System | HEK293 cells |
| Tag | N-terminal his-tag |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of ∼22 kDa. Due to the abundant glycosylation, it migrates as approximately ∼25-35 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one major ∼22 kDa band. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay |
| Endotoxin Level | < 0.5 EU per µg of the protein as determined by the LAL method |
| Research Area | Angiogenesis, Antibody Drug Development, Drug Antibody (DA) Detection, Protein Therapeutics, Receptor-Ligand Binding |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under denature conditions.
Lane 5: Deglycosylation enzymes only without target proteins.
Binding Function
Activity
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
