Recombinant Human Caspase-3 protein derived from E.coli
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P42574 |
| Gene Id | 836 |
| Gene Symbols | CASP3 |
| Protein Name / Synonyms | Cysteine protease CPP32, Caspase-3 |
| Expressed Region | Ser29-His277 |
| Expression System | E. coli |
| Tag | N-terminal 6x histidine tag |
| Purity | > 90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | 30.7 kDa |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in 20 mM NaH2PO4 (pH 8.0), 0.5 M NaCl, 1000mM imidazole, 6 M urea. |
| Concentration (lot specific) | Lot specific (see the label on the vial) |
| Preservative | None |
| Activity | The activity of the PAC-1-activated Caspase-3 was measured by its ability to cleave Ac-DEVD-pNA. The specific activity is > 4,000 pmol/min/µg. |
| Endotoxin Level | Not determined |
| Research Area | Apoptosis, Cancer, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Disclaimer
"Antigen Grade" is not a formally regulated industry standard, but it is commonly used to indicate that a protein is intended for antigen-based applications rather than functional assays.
Antigen Grade proteins are recombinant proteins purified under denaturing conditions (e.g., in urea) and may not retain their native conformation or biological activity. These proteins are suitable for antigen-based applications such as antibody generation, ELISA plate coating, Western blotting, and other immunological assays, but are not recommended for functional or activity assays.
Suitable Applications:
✔ Antibody production (immunogen)✔ ELISA coating antigen
✔ Western blot positive control
✔ Dot blot
✔ Epitope mapping
✔ Antibody screening, etc.
Not Recommended:
✘ Enzyme activity assays✘ Ligand-receptor binding assays (unless validated)
✘ Cell-based functional assays
✘ SPR/BLI kinetic studies requiring native folding
Why "Antigen Grade"?
Many bacterial proteins expressed as inclusion bodies are purified in 6–8 M urea. Although they can often be refolded, proteins sold in urea are generally not guaranteed to be correctly refolded or biologically active. "Antigen Grade" proteins are suitable for immunological applications but may not be appropriate for assays requiring native protein function.
