Recombinant Human erbB-3, N-terminal extracellular domain, derived from the transfected human HEK293 cells
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg, 250 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P21860 |
| Gene Id | 2065 |
| Gene Symbols | ERBB3 |
| Protein Name / Synonyms | Receptor Tyrosine-Protein Kinase erb-3, V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 3, C-erb-3, LCCS2, c-erbB3, erbB3-S, p180-ErbB3, HER3, p45-sErbB3, p85-sErbB3, Lethal Congenital Contracture Syndrome 2, MDA-BF-1, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3 |
| Expressed Region | Ser20-Thr643 |
| Expression System | HEK293 cells |
| Tag | N-terminal 6x histidine tag |
| Purity | > 90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | The recombinant human ERBB3/Her3 extracellular domain has a calculated molecular mass of 71 kDa. Due to the abundant glycosylation, it migrates as approximately 100 kDa protein bands (confirmed by Western blotting) in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
| Endotoxin Level | Not determined |
| Research Area | Cardiovascular Disease, Cancer, HER/ErbB Signaling |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa).
Lane 2: Untreated protein under reducing conditions.
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C.Ã For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
