Biotin-conjugated recombinant human HE4 protein, derived from transfected HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | Q14508 |
| Gene Id | 10406 |
| Gene Symbols | WFDC2 |
| Protein Name / Synonyms | WAP four-disulfide core domain protein 2, epididymal secretory protein E4, WAP5 |
| Expressed Region | Glu31-Phe124 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | N-terminal 8x histidine tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | The predicted size of HE4 protein is 12 kDa. The affinity purified recombinant human HE4 showed two bands: the major band is about 20 kDa, and the minor band is about 12 kDa. Both bands were confirmed by Western blotting using anti-HE4 antibody. The 20-kDa band may represent N-glycosylated form (Ronny Drapkin, et al. Cancer Res 2005, 65(6): 2162-9). |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS. |
| Concentration (lot specific) | Determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
| Endotoxin Level | Not determined |
| Protein Type | Recombinant Protein |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
