Recombinant human HIF-1-alpha derived from E.coli
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q16665 |
| Gene Id | 3091 |
| Gene Symbols | HIF1A |
| Protein Name / Synonyms | Hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) |
| Expressed Region | Leu400-Asn826 |
| Expression System | E. coli |
| Tag | N-terminal 6x histidine tag |
| Purity | > 85% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | 49 kDa |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
| Endotoxin Level | Not determined |
| Research Area | Angiogenesis, Cardiovascular Disease, Inflammation, HIF-1 alpha Signaling, Autophagy, Hypoxia Biology |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
