Recombinant human Hu C protein (ELAVL3), derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q14576 |
| Gene Symbols | ELAVL3 |
| Protein Name / Synonyms | ELAV-like protein 3, Hu-antigen C, Hu C, Paraneoplastic cerebellar degeneration-associated antigen. |
| Expressed Region | Val2-Ala367 |
| Expression System | HEK293 cells |
| Tag | N-terminal His-tag |
| Purity | >85% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 40 kDa. Due to the abundant glycosylation, it migrates as approximately 45 kDa major protein band and 30 kDa minor band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. The small 30 kDa band is likely the cleaved products. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay |
| Endotoxin Level | <0.5 EU per ug of the protein as determined by the LAL method |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: protein standard ladder (kDa).
Lane 2: untreated protein.
Lane 3: treated protein with deglycosylation enzymes under native conditions.
Lane 4: treated protein with deglycosylation enzymes under denature conditions.
Stability & Storage
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.