Biotin-conjugated recombinant human interleukin-17A (IL-17A) protein, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q16552 |
| Gene Symbols | IL17A |
| Protein Name / Synonyms | Interleukin-17A, Interleukin 17A, Interleukin 17 (Cytotoxic T-Lymphocyte-Associated Serine Esterase 8), CTLA-8, IL-17A, CTLA8, IL-17, IL17. |
| Expressed Region | Gly24-Ala155 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | N-terminal His-tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 18 kDa. Due to the abundant glycosylation, it migrates as approximately 18 and 22 kDa major protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. The 22-kDa protein band was glycosylated form. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay |
| Endotoxin Level | <0.5 EU per ug of the protein as determined by the LAL method |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: protein standard ladder (kDa).
Lane 2: untreated protein.
Lane 3: treated protein with deglycosylation enzymes under native conditions.
Lane 4: treated protein with deglycosylation enzymes under denature conditions.
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant IL17A can bind IL17R-Fc with EC50 at 54.44 ng/mL.
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.