Recombinant Human IL-9 Protein, HEK293 Supernatant
| Size | 200 µL |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P15248 |
| Gene Id | 3578 |
| Gene Symbols | IL9 |
| Protein Name / Synonyms | Interleukin-9 |
| Expressed Region | Gln19-Ile144 |
| Expression System | Human |
| Tag | N-terminal 6x histidine tag |
| Purity | Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80°C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control. |
| Molecular Weight (kDa) | One major band ~25 kDa in Western blotting rather than the predicted size of 14 kDa. The size increase was probably caused by the post-translational modifications or other unknown interaction of the expressed recombinant proteins. |
| Format | Pink liquid |
| Formulation | Culture supernatant of transfected HEK293 cells |
| Preservative | None |
| Endotoxin Level | Not determined |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
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Recombinant Human IL-9 Protein, HEK293 Supernatant
