Recombinant human insulin-like growth factor-binding protein-like 1/IGFBPL1 protein, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q8WX77 |
| Gene Symbols | IGFBPL1 |
| Protein Name / Synonyms | Human Insulin-like Growth Factor-binding Protein-like 1/IGFBPL1 Protein, IGFBPL1, Insulin-like growth factor-binding protein-like 1, IGFBP-related protein 10. |
| Expressed Region | Leu26-Met278 |
| Expression System | HEK293 cells |
| Tag | N-terminal His-tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant human IGFBPL1 protein has a calculated molecular mass of 26 kDa. Due to the glycosylation sites, it migrates as approximately 30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Determined by BCA protein assay |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Research Area | Cancer |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions
Lane 3: Treated protein with deglycosylation enzymes under native conditions
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Activity
Measured in a cell proliferation assay using HeLa human cervical epithelial carcinoma cells. Recombinant IGFBPL1 can inhibit cell proliferation with EC50 at 4.9 µg/mL.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
