Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4)
Protein Name / Synonyms
Human Interleukin-7 Protein, Interleukin 7, IL-7, Interleukin-7.
Accession Number
P13232-1
Gene Symbols
IL7
Concentration (lot specific)
Determined by BCA protein assay
Purity
>90%
Purity Determined By
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water.
Expressed Region
Asp26-His177
Expression System
Human embryonic kidney 293 (HEK293) cells
Molecular Weight
Recombinant human IL-17 protein has a calculated molecular mass of 17 kDa. Due to 3 glycosylation sites, it migrates as approximately 30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Purification
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
SDS-PAGE Image
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated. Lane 1: Protein standard ladder (kDa) Lane 2: Untreated protein under reducing conditions Lane 3: Treated protein with deglycosylation enzymes under native conditions Lane 4: Treated protein with deglycosylation enzymes under reducing conditions. Lane 5: Deglycosylation mixture only without target proteins.
Stability & Storage
Shipping
Ice packs
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20 °C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 °C. For long term storage, it is recommended to make appropriate aliquots and store at -80 °C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
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