Recombinant human matrix metalloproteinase-9
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Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P14780 |
| Gene Id | 4318 |
| Gene Symbols | MMP9 |
| Protein Name / Synonyms | Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, Matrix Metallopeptidase 9, CLG4B, Matrix Metallopeptidase 9, Gelatinase B, Macrophage Gelatinase, Type V Collagenase, MandP2. |
| Expressed Region | Ala20-Asp707 |
| Expression System | HEK293 cells |
| Tag | N-terminal histidine tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of ∼75 kDa. Due to the abundant glycosylation, it migrates as approximately ∼120 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ∼75 kDa band. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | 25 mM Tris-HCl pH 7.5, 150 mM NaCl, 5 mM CaCl₂, 10 µM ZnCl₂, 0.01% Brij-35, 20% glycerol, 1% mannitol, 5% trehalose |
| Concentration (lot specific) | Determined by Pierce BCA protein assay kit |
| Preservative | None |
| Endotoxin Level | Not determined |
| Research Area | Cardiovascular Disease, Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1, protein standard ladder (kDa).
Lane 2, untreated protein.
Lane 3, treated protein with deglycosylation enzymes under native conditions.
Lane 4, treated protein with deglycosylation enzymes under denature conditions.
Lane 5, deglycosylation enzymes only without target proteins.
Stability & Storage
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
