Recombinant Human Proliferating Cell Nuclear Antigen (PCNA)
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | AAX43156 |
| Gene Symbols | PCNA |
| Protein Name / Synonyms | Proliferating cell nuclear antigen, PCNA, DNA polymerase delta auxiliary protein, cyclin, proliferating cell nuclear antigen. |
| Expressed Region | Phe2-Leu262 |
| Expression System | E. coli |
| Tag | N-terminal 6x histidine tag |
| Purity | > 95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | 29 kDa |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered phosphate-buffered saline (PBS) with protein concentration at 0.8 mg/mL. |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1X Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers. |
| Endotoxin Level | Not determined |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant PCNA (Code: 230-00030) can bind rabbit anti-PCNA antibody with EC50 at 24.1 ng/mL.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
