Recombinant Human PDCD1, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 50 µg, 200 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q15116 |
| Gene Id | 5133 |
| Gene Symbols | PD1, PDCD1 |
| Protein Name / Synonyms | Programmed cell death protein 1 (Protein PD-1) (hPD-1) (CD antigen CD279), Programmed cell death protein 1, Protein PD-1, hPD-1, Programmed Cell Death 1, Systemic Lupus Erythematosus Susceptibility 2, HPD-1, PD1, Programmed Cell Death 1 Protein, CD279 Antigen, CD279, HPD-L, HSLE1, SLEB2, PD-1 |
| Expressed Region | Pro21-Val170 |
| Expression System | HEK293 cells |
| Tag | N-terminal histidine tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein has a calculated molecular weight of about 16 kDa. Due to the abundant glycosylation, it migrates as approximately 30-40 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by Pierce BCA protein assay kit |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water. |
| Endotoxin Level | Not determined |
| Research Area | Cancer, Antibody Drug Development, Drug Antibody (DA) Detection, Protein Therapeutics, Receptor-Ligand Binding, Autoimmune Disease, Cancer Immunology |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa).
Lane 2: Untreated protein under reducing conditions.
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Activity
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
