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ISO 13485:2016 cGMP
Recombinant Human PDGF BB HOMODIMER
Catalog #: DS-01-0489
Product Description
Specifications
| Size | 10 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Protein Name / Synonyms | PDGF, |
| Purity | >98% by SDS PAGE and HPLC analysis |
| Molecular Weight (kDa) | 24.3kD (218 amino acid sequence) |
| Format | Purified recombinant protein - lyophilized |
| Formulation | 10 mM Acetic Acid |
| Concentration (lot specific) | Total protein concentration ~0.1 mg/ml after reconstitution. |
| Preservative | None present |
| Recommended Applications | ELISA (0.2-0.4ng/well), Functional Assays (0.2ng/ml-10ng/ml), Western Blotting (1.5-3.0ng/lane), ELISA (0.2-0.4ng/well), Functional Assays (0.2ng/ml-10ng/ml), Western Blotting (1.5-3.0ng/lane) |
| Endotoxin Level | <0.1ng/ug |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
DS-01-0489 is an E. coli-derived recombinant protein corresponding to mature full-length human Platelet-Derived Growth Factor BB homodimer (PDGF-BB), a potent mitogen for cells of mesenchymal origin. PDGF is composed of a dimer of two chains, known as the A chain and B chain. The dimer may present as either AA or BB homodimer, or as an AB heterodimer.
DS-01-0489 has an ED50 of 1.0-3.0 ng/ml as determined by stimulation of the proliferation of Balb/c 3T3 cells.
DS-01-0489 has an ED50 of 1.0-3.0 ng/ml as determined by stimulation of the proliferation of Balb/c 3T3 cells.
Specifications
Size
10 ug
Stability & Storage
Storage/Stability
Prior to reconstitution store at +4°C. Following reconstitution store at -20°C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.