Recombinant human purine nucleoside phosphorylase/PNP, with a C-terminal His-tag. Derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 25 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P00491 |
| Gene Id | 4860 |
| Gene Symbols | PNP, NP |
| Protein Name / Synonyms | Purine nucleoside phosphorylase (PNP); Inosine phosphorylase; Inosine-guanosine phosphorylase |
| Expressed Region | Glu2-Ser289 |
| Expression System | HEK293 cells |
| Tag | C-terminal His-Avi tag |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining. See gel image below. |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of ~32 kDa. |
| Format | Liquid |
| Formulation | Filtered solution in 1x PBS (pH 7.4) with 1% mannitol and 5% trehalose |
| Concentration (lot specific) | Lot specific. See vial label, determined by Pierce BCA protein assay reagent. |
| Endotoxin Level | < 0.5 EU per μg of the protein as determined by the LAL method |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Storage/Stability
Upon arrival, the liquid protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
