Recombinant human Prolactin (PRL) protein, derived from transfected HEK293 cells
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg, 250 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P01236 |
| Gene Id | 5617 |
| Gene Symbols | PRL |
| Protein Name / Synonyms | Prolactin, PRL |
| Expressed Region | Leu29-Cys227 |
| Expression System | HEK293 cells |
| Tag | N-terminal 8x histidine tag |
| Purity | > 95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | The predicted size of prolactin is 25 kDa. The affinity purified recombinant human prolactin showed two bands: the major band is about 25 kDa, and the minor band is about 27 kDa. Both bands were confirmed by Western blotting using anti-prolactin antibody. The 27-kDa band may represent N-glycosylated and phosphorylated form. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS. |
| Concentration (lot specific) | Determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. Fully dissolve in freshly prepared 4 mM HCl, then add equal volume of 1x PBS (pH 7.4) to bring the concentration to 0.5 mg/mL. |
| Endotoxin Level | Not determined |
| Research Area | Angiogenesis, Cytoskeletal Reorganization |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa).
Lane 2: Untreated protein under reducing conditions.
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Activity
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
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