Recombinant Human Ras GTPase-activating protein 1/RASA1, transfected HEK293 cell culture supernatant
| Size | 200 µL |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P20936 |
| Gene Id | 5921 |
| Gene Symbols | RASA1 |
| Protein Name / Synonyms | Ras GTPase-activating protein 1, GAP, GTPase-activating protein, RasGAP, Ras p21 protein activator, p120GAP, RAS P21 Protein Activator (GTPase Activating Protein) 1, CMAVM, RASA, PKWS, Capillary Malformation-Arteriovenous Malformation, Triphosphatase-Activating Protein, P120RASGAP, CM-AVM |
| Expressed Region | Met2-Arg1047 |
| Expression System | HEK293 cells |
| Tag | N-terminal 6x histidine tag |
| Purity | Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80°C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control. |
| Molecular Weight (kDa) | Recombinant protein has a calculated molecular mass of 100. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs). |
| Format | Pink liquid |
| Formulation | Culture supernatant of transfected HEK293 cells |
| Preservative | None |
| Endotoxin Level | Not determined |
| Research Area | Cancer, Ras/Rap Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Recombinant Human Ras GTPase-activating protein 1/RASA1, transfected HEK293 cell culture supernatant