Recombinant Human alpha-1-Antitrypsin (AAT) / SERPINA1, expressed in E. coli
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P01009 |
| Gene Id | 5265 |
| Gene Symbols | SERPINA1 |
| Protein Name / Synonyms | Alpha-1-antitrypsin, Serpin A1, AAT, PI1 |
| Expressed Region | Glu25-Lys418 |
| Expression System | E. coli |
| Tag | N-terminal 6x histidine tag |
| Purity | > 95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | 47 kDa |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered PBS (pH 7.4) containing 1% sucrose solution with the protein concentration of 139 µg/ml |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.1~ 1 mg/mL with sterile ice-cold deionized water or 1X❏, Phosphate Buffered Saline (PBS). This solution can then be further diluted into other aqueous buffers. |
| Endotoxin Level | Not determined |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human SERPINA1 (Code: 230-00209) can bind rabbit anti-human SERPINA1 with EC50 at 10.7 ng/mL.
Measured by its binding ability in a functional ELISA. Immobilized recombinant SERPINA1 at 1 µg/mL (100 μL/well) can bind Human KRT8 with EC50 at 1.5 µg/mL.
Measured by its binding ability in a functional ELISA. Immobilized recombinant SERPINA1 at 1 µg/mL (100 μL/well) can bind Human KRT8 with EC50 at 1.5 µg/mL.
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
