Recombinant Human Serpin B3 (SCCA1) Protein, HEK293

Recombinant Human Serpin B3 (SCCA1), derived from the transfected human HEK293 cells

Product Description

Specifications

Size	10 µg, 50 µg, 100 µg
Estimated Lead Time	1-2 business days
Species	Human
Accession Number	P29508
Gene Id	6317
Gene Symbols	SERPINB3
Protein Name / Synonyms	Serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 3, SCCA-1, SCCA1, HsT1196, SCC, SCCA-PD, Serine (Or Cysteine) Proteinase Inhibitor, Clade B (Ovalbumin), Member 3, T4-A, Protein T4-A, Serpin B3, Squamous Cell Carcinoma Antigen 1, SCCA
Expressed Region	Asn2-Pro390
Expression System	HEK293 cells
Tag	N-terminal 6x histidine tag
Purity	> 90%
Purity Determined By	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)	The recombinant human SERPINB3 has a calculated molecular mass of 46 kDa. Due to the abundant glycosylation, it migrates as an approximately 55 kDa protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Format	Lyophilized powder
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS
Concentration (lot specific)	Determined by BCA protein assay kit (Thermo Scientific)
Preservative	None
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level	Not determined
Shipping Type	Ambient temperature
Storage	-20°C

SDS-PAGE Image

Activity

Storage/Stability

Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.