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ISO 13485:2016 cGMP
Recombinant Human TGF BETA 1
Catalog #: 229-20178
Product Description
Specifications
| Size | 10 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Accession Number | P01137 |
| Gene Id | 7040 |
| Gene Symbols | TGFB1,TGFB |
| Protein Name / Synonyms | TGF Beta 1, Transforming Growth Factor Beta |
| Preparation | Recombinant protein prepared from CHO cells. |
| Purity | >98% by SDS PAGE and HPLC analysis |
| Molecular Weight (kDa) | 25.0 kDa (2x identical 112 amino acid polypeptide chains) |
| Format | Purified recombinant protein - lyophilised |
| Concentration (lot specific) | 0.1 mg/ml after reconstitution |
| Preservative | None present |
| Activity | The ED50, determined by the ability of TGF beta 1 to inhibit the mouse IL-4-dependent proliferationof mouse HT-2 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg |
| Recommended Applications | Functional Assays |
| Reconstitution | Reconstitute with 100ul distilled water. Further dilutions should be made in buffer containing a carrier protein such as 0.1% BSA. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. DO NOT VORTEX. |
| Endotoxin Level | < 0.1 ng/ug |
| Research Area | Angiogenesis, Cardiovascular Disease, Cancer, Inflammation |
| Recombinant or Native | Recombinant |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Carrier Free
Yes
Storage
Prior to reconstitution store at -20°C. Following reconstitution store at -20°C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.