Recombinant Human VEGF-A Protein

Recombinant human vascular endothelial growth factor A

Product Description

Specifications

Size	10 µg, 50 µg, 100 µg
Estimated Lead Time	1-2 business days
Species	Human
Accession Number	P15692-4
Gene Symbols	VEGF
Protein Name / Synonyms	Vascular Endothelial Growth Factor A, Vascular Permeability Factor, VEGF, VPF, Vascular Endothelial Growth Factor, VEGF-A, MVCD1.
Expressed Region	Ala27-Arg191
Expression System	HEK293 cells
Tag	N-terminal histidine tag
Purity	>95%
Purity Determined By	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)	Recombinant protein has a calculated molecular weight of about 19 kDa. Due to the abundant glycosylation, it migrates as approximately 22-30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Format	Lyophilized powder
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS
Concentration (lot specific)	Determined by Pierce BCA protein assay kit
Preservative	None
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
Endotoxin Level	Not determined
Research Area	Angiogenesis, Cardiovascular Disease, Antibody Drug Development, Drug Antibody (DA) Detection, Protein Therapeutics, Receptor-Ligand Binding, Vascular Biology
Shipping Type	Ambient temperature
Storage	-20°C

SDS-PAGE Image

Activity

Stimulating cell growth significantly. Measured in a cell proliferation assay using A549 human lung carcinoma cells.

Storage/Stability

Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.