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Recombinant Mouse INTERLEUKIN-1 BETA
Catalog #: 229-20302
Purified recombinant murine IL-1 beta, expressed in E. coli
Product Description
Specifications
| Size | 10 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse |
| Protein Name / Synonyms | IL-1 BETA, |
| Expression System | E. coli |
| Purity | >98% by SDS PAGE and HPLC analysis |
| Molecular Weight (kDa) | 17.5kD (153 amino acid residues) |
| Format | Purified recombinant protein - lyophilized |
| Concentration (lot specific) | ~0.1mg/ml after reconstitution |
| Preservative | None present |
| Activity | The ED50 as determined by the dose-dependent stimulation of murine D10S cells is <0.002ng/ml, corresponding to a specific activity of > 5x108 units/mg. |
| Recommended Applications | ELISA (0.2-0.4ng/well), Functional Assays, Western Blotting (1.5-3.0ng/lane), ELISA (0.2-0.4ng/well), Functional Assays, Western Blotting (1.5-3.0ng/lane) |
| Endotoxin Level | <0.1ng/ug |
| Research Area | Inflammation |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Introduction
Interleukin-1 beta (IL-1 beta), is a 17.5 kDa proinflammatory, cell-associated cytokine, produced by monocytes, tissue macrophages, keratinocytes and other epithelial cells. IL-1 beta promotes the proliferation of memory T cells and cytokine production. It attenuates regulatory T cell function and enables CD4+CD25- autoreactive effectors to avoid suppression. IL-1 beta signals downstream of myc and is required for angiogenesis and invasiveness of tumour cells. IL-1 beta is directly involved in neuronal injury in neurodegenerative disorders, and stimulates mitogenic FGF-like activity, bone resorption and promotes the release of collagenase and prostaglandin from synovial cells.
Specifications
Size
10 ug
Stability & Storage
Storage/Stability
Prior to reconstitution store at +4°C.
After reconstitution store at -20°C only.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.
After reconstitution store at -20°C only.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.