Recombinant mouse TFF2, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Accession Number | Q9QX97 |
| Gene Symbols | TFF2 |
| Protein Name / Synonyms | Tff2 protein, Trefoil Factor 2, TFF2 |
| Expressed Region | Glu24-Tyr129 |
| Expression System | HEK293 cells |
| Tag | N-terminal 6x histidine tag and C-terminal mouse IgG Fc tag |
| Purity | > 90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant mouse TFF2 has a calculated molecular mass of 42 kDa (including a C-terminal mouse IgG Fc tag, 27 kDa). Due to the abundant glycosylation, it migrates as approximately 45-50 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
| Endotoxin Level | Not determined |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa).
Lane 2: Untreated protein under reducing conditions.
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Lane 5: Deglycosylation mixture only without target proteins.
Stability & Storage
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
