Product Description

Specifications

Size	10 µg, 50 µg
Estimated Lead Time	1-2 business days
Species	Sus scrofa
Accession Number	P16545
Gene Id	397491
Gene Symbols	IGF1
Protein Name / Synonyms	Insulin-like growth factor I (IGF-I) (Somatomedin), Insulin-like growth factor I, IGF-I, Somatomedin.
Expressed Region	Gly49-Ala118
Expression System	HEK293 cells
Tag	N-terminal histidine tag and C-terminal Fc tag
Purity	>95%
Purity Determined By	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)	Recombinant protein has a calculated molecular weight of about 40 kDa, including C-terminal Fc-tag.
Format	Lyophilized powder
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS
Concentration (lot specific)	Determined by Pierce BCA protein assay kit
Preservative	None
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.
Endotoxin Level	Not determined
Research Area	IGF Signaling
Shipping Type	Ambient temperature
Storage	-20°C

SDS-PAGE Image

Activity

Stimulating cell growth significantly. Measured in a cell proliferation assay using MCF‑7 human breast cancer cells.

Storage/Stability

Upon arrival, the lyophilized protein may be stored for 2 weeks at 4^°C. For long term storage, it is recommended to store desiccated below -20 ^°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20 ^°C.Ã For long term storage, it is recommended to make appropriate aliquots and store at -80 ^°C. Avoid repeated freeze-thaw cycles.