Recombinant Staphylococcus aureus Penicillin Binding Protein 2 (PBP2a), expressed in E. coli
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Product Description
Specifications
| Size | 50 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Staphylococcus spp |
| Specificity | Staphylococcus Aureus Subsp. |
| Accession Number | Q53707 |
| Gene Symbols | MECA |
| Protein Name / Synonyms | PBP2a, Penicillin binding protein 2 |
| Expressed Region | Gly19-Glu668 |
| Expression System | E. coli |
| Tag | N-terminal 6x histidine tag |
| Purity | > 90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | 74 kDa |
| Format | Lyophilized powder |
| Formulation | Lyophilized from 0.2 µm filtered solution in PBS (pH 7.4). |
| Concentration (lot specific) | Determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water. |
| Endotoxin Level | Not determined |
| Research Area | Infectious Disease, Antimicrobial Resistance |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Introduction
Penicillin-Binding Protein 2a (PBP2a) is an altered penicillin-binding protein found in methicillin-resistant Staphylococcus aureus (MRSA) strains. It has a low affinity for β-lactam antibiotics, allowing MRSA to continue synthesizing its cell wall even in the presence of these antibiotics, thereby conferring resistance. The mecA gene encodes PBP2a, and its acquisition is a key factor in the development of methicillin resistance in S. aureus. Understanding the structure and function of PBP2a is crucial for developing new strategies to combat MRSA infections.
Reference: https://pubmed.ncbi.nlm.nih.gov/15226303/
SDS-PAGE Image
Stability & Storage
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
