Recombinant SARS CoV-2 Omicron XBB.1 S1RBD with a C-terminal His-tag, derived from the transfected human HEK293 cells.
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Figure 1. Deglycosylation analysis of purified recombinant proteins. Lane 1, protein ladder (kDa). Lane 2, glycosylated protein (delivered to customers). Lanes 3 and 4, deglycosylated proteins under native and denature conditions respectively.
| Size | 10 µg |
| Estimated Lead Time | 1-2 business days |
| Species | SARS-CoV-2 |
| Accession Number | QHD43416 |
| Gene Symbols | S |
| Protein Name / Synonyms | SARS-CoV-2 Spike Protein, S1 Subunit, S protein, spike protein |
| Expressed Region | Arg319-Phe541. 22 site mutation: G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H. |
| Expression System | HEK293 cells |
| Tag | C-terminal histidine tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 35 kDa major protein band in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by Bradford protein assay |
| Endotoxin Level | <0.5 EU per µg of the protein as determined by the LAL method |
| Recombinant or Native | Recombinant |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Storage / Stability | Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles. |

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Figure 1. Deglycosylation analysis of purified recombinant proteins. Lane 1, protein ladder (kDa). Lane 2, glycosylated protein (delivered to customers). Lanes 3 and 4, deglycosylated proteins under native and denature conditions respectively.