Recombinant SARS-CoV-2 S1 subunit protein, C-terminal CendR domain with an N-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 50 µg, 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | SARS-CoV-2 |
| Accession Number | QHD43416 |
| Gene Symbols | S |
| Protein Name / Synonyms | Spike protein, S Protein, S1 Subunit, CendR Domain |
| Expressed Region | Asn542-Arg685 |
| Expression System | HEK293 cells |
| Tag | N-terminal his-tag |
| Purity | >95 % |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of ∼18 kDa. Due to the abundant glycosylation, it migrates as approximately ∼25-35 kDa protein smear bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. After deglycosylation under native and denature conditions, the protein presented as one reduced ∼18 kDa band. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay |
| Endotoxin Level | < 0.5 EU per µg of the protein as determined by the LAL method |
| Research Area | Infectious Disease, COVID-19 |
| Shipping Type | Blue ice |
| Storage | -20°C |
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein
Lane 3: Treated protein with deglycosylation enzymes under native conditions.
Lane 4: Treated protein with deglycosylation enzymes under denature conditions.
Lane 5: Deglycosylation enzymes only without target proteins.
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant S1 CendR can bind Human Nrp1 with EC50 at 225.3 ng/mL.
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
