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Ribonuclease T1, Chromatographically Purif.
Catalog #: 255-10076
Highly purified, microbial (non-mammalian) RNase prepared with non-animal components. Supplied as a solution in 2.8 M ammonium sulfate.
Product Description
Specifications
| Size | 100 KU, 500 KU |
| Estimated Lead Time | 1-2 weeks |
| Accession Number | P00651 |
| Expression System | Aspergillus oryzae |
| Molecular Weight (kDa) | Molecular Weight11 kDa (Egami et al. 1964) |
| Activity | ≥300,000 units per mg protein |
| Recommended Applications | Cleavage enzyme for the determination of structure, nearest neighbor frequencies, and sequence of RNA (Miura and Egami 1960, Rushizky and Sober 1962, Miura 1964b, Holley et al. 1965, Madison and Kung 1967, and Neelon et al. 1967) Preparation of nucleoside, Cleavage enzyme for the determination of structure, nearest neighbor frequencies, and sequence of RNA (Miura and Egami 1960, Rushizky and Sober 1962, Miura 1964b, Holley et al. 1965, Madison and Kung 1967, and Neelon et al. 1967) Preparation of nucleoside 2', 3'-cyclic phosphates Synthesis of oligonucleotides Removal of RNA from DNA preparations (Saito and Miura 1963) Removal of poly(A) sequences from mRNA in the presence of oligo(dT) Removal of mRNA during single-strand cDNA synthesis RNA fingerprinting Oligodeoxyribonucleotide-directed cleavage of RNA |
| Source | Aspergillus oryzae |
| Shipping Type | Blue ice |
| Storage | 0-8°C |
Description
Other Information
Extinction Coefficient
17,690 cm-1 M-1 E1%,278 Equals 2.9 (Egami et al. 1964)
Isoelectric
2.9 (Egami et al. 1964) 4.12 (Theoretical)
CATH
Class: Alpha Beta Architecture: Roll Topology: Nuclear Transport Factor 2; Chain A
CAS Registry Number
C.A.S.: 9026-12-4
Storage Conditions
Store at 0-8°C