Product Features
- Quick and sensitive RNAse activity measurement
- Compatible with Real-Time PCR instruments
- Selective for RNAse, minimal DNAse cross-reactivity
- Reliable detection down to 1.6 pg per reaction
- Simple, straightforward assay procedure
Kit Components
- ssRNA Probe
- Positive Control
- Cleavage Buffer
- Sample Diluent
- Optical Seal
Additional Materials Required
- Vortex Mixer
- Tabletop centrifuge
- Pipettes and nuclease-free pipette tips (barrier tips recommended)
- Sterile RNase/DNAse free cotton swabs
- Nuclease free dH2O
- Plate reader or PCR thermocycler
- Nuclease free 96-Well PCR plate
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed.
- Add Master Mix to each well and mix.
- Add controls and samples to the designated wells.
- Measure fluorescence using a compatible instrument.
Storage/Stability
Store the kit components according to storage temperatures for up to 3 months. Protect ssRNA Probe from light. Kit must be used within expiry period to ensure optimal performance. Do not mix reagents from different kit lots.