Sandwich ELISA Kits

Quantitative, Sandwich ELISAs for Detection of Soluble Factors

Sandwich ELISA is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of soluble proteins in biological fluids. RayBiotech sandwich ELISA kits are manufactured in Peachtree Corners, GA, validated with 5 different sample matrices* and backed by a 100% satisfaction guarantee. Under a large and highly experienced team of R&D scientists, our sandwich ELISA catalog has grown to cover 3000 target analytes and 15 species. When you buy directly from RayBiotech and our authorized distributors, you will never pay 3rd party markups, giving you access to the most competitive prices available. This, along with 3000+ literature citations, has made RayBio® sandwich ELISA kits some of the most trusted in the industry.

*EDTA plasma, citrate plasma, heparin plasma, serum, cell culture medium.

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How It Works

Sandwich ELISA Detection

RayBio® sandwich ELISAs employ specific capture antibodies coated on a 96-well plate. Standards and samples are pipetted into the wells; the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the biotin-labeled detection antibody is then added. After washing away the unbound biotinylated antibodies, HRP-conjugated streptavidin is pipetted to the wells, followed by a colorimetric substrate solution. The intensity of color development in the wells is proportional to the amount of target protein bound.


  • Excellent for follow up/confirmation of antibody array data
  • Pre-coated 96 well plate
  • Strip plate format minimizes waste
  • Complete kit includes all necessary reagents
  • Highly sensitive and specific
  • Reproducible and reliable
  • Optimized for serum, plasma, urine and cell culture medium
  • Hundreds of choices

Contents of Kit

  • 96-well pre-coated strip plate (12x8)
  • Wash Buffer Concentrate (20x)
  • Standard
  • Sample Diluent Buffers
  • Biotin-labeled Detection Antibody
  • HRP-Streptavidin concentrate
  • TMB One-Step Substrate Reagent
  • Stop Solution
  • Cell Lysis Buffer (for cell/tissue lysate kits only)

Why RayBiotech ELISA Kits?

  • We manufacture, not resell, ELISA kits. Right here in the USA.
  • Our ELISAs are ISO certified and validated for exceptional reproducibility. Read more about our quality control processes.
  • All ELISAs are backed by our 90-day 100% satisfaction guarantee.
  • Discounts and incentives available for publications citing our products, and for product reviews.


  • Cardenas E., Breaux K., Da Q., et ai. Platelet Munc13-4 regulates hemostasis, thrombosis and airway inflammation. Haematologica. 2018 Apr 19. pii: haematol.2017.185637. doi: 10.3324/haematol.2017.185637. [Epub ahead of print] [view publication]
    Product: Mouse PF-4 ELISA
    Sample Type: Platelet Releasate
  • Chandan R., Banerjee R. Pro-apoptotic liposomes-nanobubble conjugate synergistic with paclitaxel: a platform for ultrasound responsive image-guided drug delivery. Sci Rep. 2018 Feb 8;8(1):2624. doi: 10.1038/s41598-018-21084-8. [view publication]
    Products: Mouse IFN-gamma ELISA | Mouse IL-6 ELISA | Mouse TNF-alpha ELISA
    Sample Type: Conditioned Media
  • Chen D., Chen Y., Chen H., et al. Human parvovirus B19 nonstructural protein NS1 activates NLRP3 inflammasome signaling in adult‑onset Still's disease. Mol Med Rep. 2018 Feb;17(2):3364-3371. doi: 10.3892/mmr.2017.8275. [view publication]
    Product: Human IL-1 beta ELISA
    Sample Type: Conditioned Media
  • Dey S., Bishayi B. Killing of S. aureus in murine peritoneal macrophages by Ascorbic acid along with antibiotics Chloramphenicol or Ofloxacin: Correlation with inflammation. Microb Pathog. 2018 Feb;115:239-250. doi: 10.1016/j.micpath.2017.12.048 [view publication]
    Products: Mouse IFN-gamma ELISA | Mouse IL-10 ELISA | Mouse IL-6 ELISA | Mouse TNF-alpha ELISA
    Sample Type: Conditioned Media
  • Etulain J., Mena H., Meiss R., et al. An optimised protocol for plateletrich plasma preparation to improve its angiogenic and regenerative properties. Scientific Reports (2018) 8:1513 | DOI:10.1038/s41598-018-19419-6 [view publication]
    Product: Human VEGF-A ELISA
    Sample Type: Conditioned Media
  • Habashy N., Abu Serie M., Attia W., Abdelgaleil S. Chemical characterization, antioxidant and anti-inflammatory properties of Greek Thymus vulgaris extracts and their possible synergism with Egyptian Chlorella vulgaris. Journal of Functional Foods , Volume 40, January 2018, Pages 317-328, [view publication]
    Product: Human TNF-alpha ELISA
    Sample Type: Conditioned Media
  • Li X., Yan X., Wang Y., et al. NLRP3 inflammasome inhibition attenuates silica-induced epithelial to mesenchymal transition (EMT) in human bronchial epithelial cells. Exp Cell Res. 2018 Jan 15;362(2):489-497. doi: 10.1016/j.yexcr.2017.12.013. [view publication]
    Product: Human IL-18 ELISA
    Sample Type: Conditioned Media
  • Linder M., Hecking M., Glitzner E., et al. EGFR controls bone development by negatively regulating mTOR-signaling during osteoblast differentiation. Cell Death Differ. 2018 Feb 14. doi: 10.1038/s41418-017-0054-7 [view publication]
    Product: Mouse IGF-2 ELISA
    Sample Type: Conditioned Media
  • Mendt M., Kamerkar S., Sugimoto H., et al. Generation and testing of clinical-grade exosomes for pancreatic cancer. JCI Insight. 2018 Apr 19;3(8). pii: 99263. doi: 10.1172/jci.insight.99263. [view publication]
    Product: Mouse IL-6 ELISA
    Sample Type: Serum
  • Mohamed AAR, Elbohi KM, Sharkawy NIE and Hassan MA. Biochemical and Apoptotic Biomarkers as Indicators of Time Elapsed Since Death in Experimentally Induced Traumatic Brain Injury. Beni-Suef University Journal of Basic and Applied Sciences, [view publication]
    Product: Rabbit IL-1 beta ELISA
    Sample Type: Serum
  • Sambataro M, Sambado L., Trevisiol E., et al. Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions. FASEB J. 2018 Feb 12:fj201701279RR. doi: 10.1096/fj.201701279RR [view publication]
    Products: Human IFN-gamma ELISA | Human IL-10 ELISA | Human IL-12 p70 ELISA | Human IL-4 ELISA | Human MCP-1 ELISA | Human TNF-alpha ELISA
    Sample Type: Plasma
  • Schumann C., Nguyen D., Norgard M., et al. Increasing lean muscle mass in mice via nanoparticle-mediated hepatic delivery of follistatin mRNA. Theranostics. 2018; 8(19): 5276–5288. Published online 2018 Oct 22. doi: 10.7150/thno.27847 [view publication]
    Products: Human Follistatin ELISA | Human GDF-8/Myostatin ELISA | Mouse Activin A ELISA
    Sample Type: Conditioned Media
  • Siqi Feng, Ya Su, Li Luo, Jing Fengchuan, Qijian Yi, Serum levels of C1q/tumor necrosis factor related protein-1 in children with Kawasaki disease, Pediatric Research accepted article preview 23 January 2018; doi: 10.1038/pr.2018.16. [view publication]
    Products: Human C1qTNF1 ELISA | Human IL-1 beta ELISA | Human IL-6 ELISA | Human TNF-alpha ELISA
    Sample Type: Serum
  • Sun J., Zhao J., Wang L., et al. Inner Filter Effect-Based Sensor for Horseradish Peroxidase and Its Application to Fluorescence Immunoassay. ACS Sens. 2018 Jan 26;3(1):183-190. doi: 10.1021/acssensors.7b00830. [view publication]
    Product: Human AFP ELISA
    Sample Type: Serum
  • Szpak D., Izem L., Verbovetskiy D., et al. αMβ2 Is Antiatherogenic in Female but Not Male Mice. J Immunol. 2018 Apr 1;200(7):2426-2438. doi: 10.4049/jimmunol.1700313. [view publication]
    Products: Mouse IL-10 ELISA | Mouse IL-12 p40/70 ELISA | Mouse IL-6 ELISA
    Sample Type: Conditioned Media
  • Willy K., Girndt M., Voelkl J., et al. Expanded Haemodialysis Therapy of Chronic Haemodialysis Patients Prevents Calcification and Apoptosis of Vascular Smooth Muscle Cells in vitro. Blood Purif. 2018;45(1-3):131-138. doi: 10.1159/000484925. [view publication]
    Product: Human GDF-15 ELISA
    Sample Type: Conditioned media
  • Zhou R., Wang X., Liu H., et al. GalNAc-Specific Soybean Lectin Inhibits HIV Infection of Macrophages through Induction of Antiviral Factors. J Virol. 2018 Feb 26;92(6). pii: e01720-17. doi: 10.1128/JVI.01720-17. [view publication]
    Products: Human MIP-1 beta ELISA | Human RANTES ELISA
    Sample Type: Conditioned Media

More references are listed per product on the individual product pages.