The RayBio® ELISA kits are in vitro enzyme-linked immunosorbent assays for the quantitative measurement of soluble proteins in a variety of species. Our extensive ELISA selection includes cytokines, growth factors, proteases, soluble receptors, apoptosis effectors, and many other soluble proteins.
For a comparison & overview of all our ELISA systems, click here.
RayBio® sandwich ELISAs employ specific capture antibodies coated on a 96-well plate. Standards and samples are pipetted into the wells; the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the biotin-labeled detection antibody is then added. After washing away the unbound biotinylated antibodies, HRP-conjugated streptavidin is pipetted to the wells, followed by a colorimetric substrate solution. The intensity of color development in the wells is proportional to the amount of target protein bound.
More references are listed per product on the individual product pages.