Sandwich ELISA Kits

Quantitative, Sandwich ELISAs for Detection of Soluble Factors in a Variety of Species

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Sandwich ELISA

The RayBiotech sandwich ELISA kits are in vitro enzyme-linked immunosorbent assays for the quantitative measurement of soluble proteins in a variety of species. Our extensive ELISA selection includes cytokines, growth factors, proteases, soluble receptors, apoptosis effectors, and many other soluble proteins.

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Features

  • Excellent for follow up/confirmation of antibody array data
  • Pre-coated 96 well plate
  • Strip plate format minimizes waste
  • Complete kit includes all necessary reagents
  • Highly sensitive and specific
  • Reproducible and reliable
  • Optimized for serum, plasma, urine and cell culture medium
  • Hundreds of choices

Contents of Kit

  • 96-well pre-coated strip plate (12x8)
  • Wash Buffer Concentrate (20x)
  • Standard
  • Sample Diluent Buffers
  • Biotin-labeled Detection Antibody
  • HRP-Streptavidin concentrate
  • TMB One-Step Substrate Reagent
  • Stop Solution
  • Cell Lysis Buffer (for cell/tissue lysate kits only)

Representative Data

Sandwich ELISA Standard Curve

Research Applications

  • Detection of quantitative protein levels in biological fluids
  • Validation of antibody array results
  • Validation of biomarker discovery studies

How It Works

Sandwich-ELISA Detection

RayBio® sandwich ELISAs employ specific capture antibodies coated on a 96-well plate. Standards and samples are pipetted into the wells; the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the biotin-labeled detection antibody is then added. After washing away the unbound biotinylated antibodies, HRP-conjugated streptavidin is pipetted to the wells, followed by a colorimetric substrate solution. The intensity of color development in the wells is proportional to the amount of target protein bound.

References

  • Cardenas E., Breaux K., Da Q., et ai. Platelet Munc13-4 regulates hemostasis, thrombosis and airway inflammation. Haematologica. 2018 Apr 19. pii: haematol.2017.185637. doi: 10.3324/haematol.2017.185637. [Epub ahead of print] [view publication]
    Product: Mouse PF-4 ELISA
    Sample Type: Platelet Releasate
  • Chandan R., Banerjee R. Pro-apoptotic liposomes-nanobubble conjugate synergistic with paclitaxel: a platform for ultrasound responsive image-guided drug delivery. Sci Rep. 2018 Feb 8;8(1):2624. doi: 10.1038/s41598-018-21084-8. [view publication]
    Products: Mouse IFN-gamma ELISA | Mouse IL-6 ELISA | Mouse TNF-alpha ELISA
    Sample Type: Conditioned Media
  • Chen D., Chen Y., Chen H., et al. Human parvovirus B19 nonstructural protein NS1 activates NLRP3 inflammasome signaling in adult‑onset Still's disease. Mol Med Rep. 2018 Feb;17(2):3364-3371. doi: 10.3892/mmr.2017.8275. [view publication]
    Product: Human IL-1 beta ELISA
    Sample Type: Conditioned Media
  • Dey S., Bishayi B. Killing of S. aureus in murine peritoneal macrophages by Ascorbic acid along with antibiotics Chloramphenicol or Ofloxacin: Correlation with inflammation. Microb Pathog. 2018 Feb;115:239-250. doi: 10.1016/j.micpath.2017.12.048 [view publication]
    Products: Mouse IFN-gamma ELISA | Mouse IL-10 ELISA | Mouse IL-6 ELISA | Mouse TNF-alpha ELISA
    Sample Type: Conditioned Media
  • Etulain J., Mena H., Meiss R., et al. An optimised protocol for plateletrich plasma preparation to improve its angiogenic and regenerative properties. Scientific Reports (2018) 8:1513 | DOI:10.1038/s41598-018-19419-6 [view publication]
    Product: Human VEGF-A ELISA
    Sample Type: Conditioned Media
  • Habashy N., Abu Serie M., Attia W., Abdelgaleil S. Chemical characterization, antioxidant and anti-inflammatory properties of Greek Thymus vulgaris extracts and their possible synergism with Egyptian Chlorella vulgaris. Journal of Functional Foods , Volume 40, January 2018, Pages 317-328, doi.org/10.1016/j.jff.2017.11.022 [view publication]
    Product: Human TNF-alpha ELISA
    Sample Type: Conditioned Media
  • Li X., Yan X., Wang Y., et al. NLRP3 inflammasome inhibition attenuates silica-induced epithelial to mesenchymal transition (EMT) in human bronchial epithelial cells. Exp Cell Res. 2018 Jan 15;362(2):489-497. doi: 10.1016/j.yexcr.2017.12.013. [view publication]
    Product: Human IL-18 ELISA
    Sample Type: Conditioned Media
  • Linder M., Hecking M., Glitzner E., et al. EGFR controls bone development by negatively regulating mTOR-signaling during osteoblast differentiation. Cell Death Differ. 2018 Feb 14. doi: 10.1038/s41418-017-0054-7 [view publication]
    Product: Mouse IGF-2 ELISA
    Sample Type: Conditioned Media
  • Mendt M., Kamerkar S., Sugimoto H., et al. Generation and testing of clinical-grade exosomes for pancreatic cancer. JCI Insight. 2018 Apr 19;3(8). pii: 99263. doi: 10.1172/jci.insight.99263. [view publication]
    Product: Mouse IL-6 ELISA
    Sample Type: Serum
  • Mohamed AAR, Elbohi KM, Sharkawy NIE and Hassan MA. Biochemical and Apoptotic Biomarkers as Indicators of Time Elapsed Since Death in Experimentally Induced Traumatic Brain Injury. Beni-Suef University Journal of Basic and Applied Sciences, doi.org/10.1016/j.bjbas.2018.01.001. [view publication]
    Product: Rabbit IL-1 beta ELISA
    Sample Type: Serum
  • Sambataro M, Sambado L., Trevisiol E., et al. Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions. FASEB J. 2018 Feb 12:fj201701279RR. doi: 10.1096/fj.201701279RR [view publication]
    Products: Human IFN-gamma ELISA | Human IL-10 ELISA | Human IL-12 p70 ELISA | Human IL-4 ELISA | Human MCP-1 ELISA | Human TNF-alpha ELISA
    Sample Type: Plasma
  • Schumann C., Nguyen D., Norgard M., et al. Increasing lean muscle mass in mice via nanoparticle-mediated hepatic delivery of follistatin mRNA. Theranostics. 2018; 8(19): 5276–5288. Published online 2018 Oct 22. doi: 10.7150/thno.27847 [view publication]
    Products: Human Follistatin ELISA | Human GDF-8/Myostatin ELISA | Mouse Activin A ELISA
    Sample Type: Conditioned Media
  • Siqi Feng, Ya Su, Li Luo, Jing Fengchuan, Qijian Yi, Serum levels of C1q/tumor necrosis factor related protein-1 in children with Kawasaki disease, Pediatric Research accepted article preview 23 January 2018; doi: 10.1038/pr.2018.16. [view publication]
    Products: Human C1qTNF1 ELISA | Human IL-1 beta ELISA | Human IL-6 ELISA | Human TNF-alpha ELISA
    Sample Type: Serum
  • Sun J., Zhao J., Wang L., et al. Inner Filter Effect-Based Sensor for Horseradish Peroxidase and Its Application to Fluorescence Immunoassay. ACS Sens. 2018 Jan 26;3(1):183-190. doi: 10.1021/acssensors.7b00830. [view publication]
    Product: Human AFP ELISA
    Sample Type: Serum
  • Szpak D., Izem L., Verbovetskiy D., et al. αMβ2 Is Antiatherogenic in Female but Not Male Mice. J Immunol. 2018 Apr 1;200(7):2426-2438. doi: 10.4049/jimmunol.1700313. [view publication]
    Products: Mouse IL-10 ELISA | Mouse IL-12 p40/70 ELISA | Mouse IL-6 ELISA
    Sample Type: Conditioned Media
  • Willy K., Girndt M., Voelkl J., et al. Expanded Haemodialysis Therapy of Chronic Haemodialysis Patients Prevents Calcification and Apoptosis of Vascular Smooth Muscle Cells in vitro. Blood Purif. 2018;45(1-3):131-138. doi: 10.1159/000484925. [view publication]
    Product: Human GDF-15 ELISA
    Sample Type: Conditioned media
  • Zhou R., Wang X., Liu H., et al. GalNAc-Specific Soybean Lectin Inhibits HIV Infection of Macrophages through Induction of Antiviral Factors. J Virol. 2018 Feb 26;92(6). pii: e01720-17. doi: 10.1128/JVI.01720-17. [view publication]
    Products: Human MIP-1 beta ELISA | Human RANTES ELISA
    Sample Type: Conditioned Media

More references are listed per product on the individual product pages.