Sandwich ELISA Kits

Quantitative, Sandwich ELISAs for Detection of Soluble Factors in a Variety of Species

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ELISA kit

The RayBio® ELISA kits are in vitro enzyme-linked immunosorbent assays for the quantitative measurement of soluble proteins in a variety of species. Our extensive ELISA selection includes cytokines, growth factors, proteases, soluble receptors, apoptosis effectors, and many other soluble proteins.

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Blue Ribbon Seal ELISA

Features

  • Excellent for follow up/confirmation of antibody array data
  • Pre-coated 96 well plate
  • Strip plate format minimizes waste
  • Complete kit includes all necessary reagents
  • Highly sensitive and specific
  • Reproducible and reliable
  • Optimized for serum, plasma, urine and cell culture medium
  • Hundreds of choices

Contents of Kit

  • 96-well pre-coated strip plate (12x8)
  • Wash Buffer Concentrate (20x)
  • Standard
  • Sample Diluent Buffers
  • Biotin-labeled Detection Antibody
  • HRP-Streptavidin concentrate
  • TMB One-Step Substrate Reagent
  • Stop Solution
  • Cell Lysis Buffer (for cell/tissue lysate kits only)

Representative Data

Sandwich ELISA Standard Curve

Research Applications

  • Detection of quantitative protein levels in biological fluids
  • Validation of antibody array results
  • Validation of biomarker discovery studies

How It Works

Sandwich-ELISA - How it works

RayBio® sandwich ELISAs employ specific capture antibodies coated on a 96-well plate. Standards and samples are pipetted into the wells; the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the biotin-labeled detection antibody is then added. After washing away the unbound biotinylated antibodies, HRP-conjugated streptavidin is pipetted to the wells, followed by a colorimetric substrate solution. The intensity of color development in the wells is proportional to the amount of target protein bound.

References

  1. Png K., Halberg N., Yoshida M., Tavazoie S. A microRNA regulon that mediates endothelial recruitment and metastasis by cancer cells. Nature. 2011 Dec 14;481(7380):190-4. doi: 10.1038/nature10661
    Species: Human
    Sample Type: Conditioned Media
  2. Marusyk A., et al. Non-cell-autonomous driving of tumour growthsupports sub-clonal heterogeneity. Nature 2014. Received 27 November 2013 Accepted 03 June 2014 Published online 30 July 2014. doi:10.1038/nature13556
    Species: Human
    Sample Type: Conditioned Media
  3. Schober A., Nazari-Jahantigh M., Wei Y., Bidzhekov K., Gremse F., et al. MicroRNA-126-5p promotes endothelial proliferation and limits atherosclerosis by suppressing Dlk1. Nature Medicine 20, 368-376 (2014)
    Species: Mouse
    Sample Type: Tissue Lysate
  4. Lu H., et al. A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages. Nature Cell Biology (2014) doi:10.1038/ncb3041
    Species: Human
    Sample Type: Conditioned Media
  5. Cao X, Li L-P, Wang Q, et al. Astrocyte-derived ATP modulates depressive-like behaviors. Nat Med. 2013 Jun;19(6):773-777.
    Species: Mouse
    Sample Type: Tissue Lysate
  6. Fulzele K, Krause DS, Panaroni C, et al. Myelopoiesis is regulated by osteocytes through Gsalpha-dependent signaling. Blood. 2013;121(6):930-939.
    Species: Mouse
    Sample Type: Serum
  7. Said N., Sanchez-Carbayo M., Smith S., and Theodorescu D.RhoGDI2 suppresses lung metastasis in mice by reducing tumor versican expression and macrophage infiltration. J Clin Invest. Apr 2, 2012; 122(4): 1503-1518. doi: 10.1172/JCI61392
    Species: Mouse
    Sample Type: Conditioned Media
  8. Wang X-A, Zhang R, Jiang D, et al. Interferon regulatory factor 9 protects against hepatic insulin resistance and steatosis in male mice. Hepatology. 2013 Aug;58(2):603-616.
    Species: Mouse
    Sample Type: Serum
  9. Chen C-C, Stairs DB, Boxer RB, et al. Autocrine prolactin induced by the Pten-Akt pathway is required for lactation initiation and provides a direct link between the Akt and Stat5 pathways. Genes & Development 2012;26(19):2154-2168. doi:10.1101/gad.197343.112.
    Species: Mouse
    Sample Type: Serum
  10. Matas-Cespedes A., Rodriguez V., Kalka S. et al. Disruption of follicular dendritic cells - follicular lymphoma crosstalk by the pan-PI3K inhibitor BKM120 (buparlisib). Clinical Cancer Research. Published online May 5 2014 doi: 10.1158/1078-0432.CCR-14-0154 [Epub ahead of print]
    Species: Human
    Sample Type: Conditioned Media
  11. Alam A, Haldar S, Thulasiram HV, et al. Novel Anti-inflammatory Activity of Epoxyazadiradione against Macrophage Migration Inhibitory Factor: INHIBITION OF TAUTOMERASE AND PROINFLAMMATORY ACTIVITIES OF MACROPHAGE MIGRATION INHIBITORY FACTOR. The Journal of Biological Chemistry 2012;287(29):24844-24861. doi:10.1074/jbc.M112.341321.
    Species: Mouse
    Sample Type: Serum
  12. Hoo SP, Loh QL, Yue Z, et al. Preparation of a soft and interconnected macroporous hydroxypropyl cellulose methacrylate scaffold for adipose tissue engineering. J Mater Chem B. 2013 May 20; [Epub ahead of print] doi:10.1039/C3TB00446E
    Species: Human
    Sample Type: Conditioned Media
  13. Schaal SM, Garg MS, Ghosh M, Lovera L, Lopez M, et al. (2012) The Therapeutic Profile of Rolipram, PDE Target and Mechanism of Action as a Neuroprotectant following Spinal Cord Injury. PLoS ONE 7(9): e43634. doi:10.1371/journal.pone.0043634
    Species: Rat
    Sample Type: Spinal Cord Tissue
  14. Liu A., Pu Y., Zheng Y., Cai H., Bin Y. Therapeutic efficacies of chitosan against Pneumocystis pneumonia of immunosuppressed rat. Parasite Immunology doi: 10.1111/pim.12117 [Epub Ahead of Print]
    Species: Rat
    Sample Type: Serum

More references are listed per product on the individual product pages.