SpeedELISA Kits

Quantitative, Sandwich ELISAs for Detection of Soluble Factors in just Three Hours


The RayBio® SpeedELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of target protein in biological sample such as serum, plasma, and cell culture supernatants.

Browse All SpeedELISA Kits

For a comparison & overview of all our ELISA systems, click here.

Blue Ribbon Seal ELISA


  • Compressed workflow with only one wash step and three incubation steps
  • Only 3 hours total processing time
  • Strip plate format minimizes waste
  • Complete kit includes all necessary reagents
  • Highly sensitive and specific
  • Reproducible and reliable
  • Optimized for serum, plasma, urine and cell culture medium
  • Excellent for follow up/confirmation of antibody array data

Contents of Kit

  • Streptavidin-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Capture Antibody
  • HRP Conjugated Detection Antibody
  • TMB One-Step Substrate

Representative Data

ELISA Standard Curve

Research Applications

  • Detection of quantitative protein levels in biological fluids
  • Validation of antibody array results
  • Validation of biomarker discovery studies

How It Works

SpeedELISA - How it works

RayBio® SpeedELISAs employ a biotinylated capture and a HRP-conjugated detection antibody which immunocaptures the sample analyte in solution. The microplate in the kit is precoated with streptavidin.

The biotinylated capture antibody/protein/HRP-conjugated detection antibody mixture is pipetted into the wells and the biotinylated target protein present in a sample is bound to the wells by the immobilized streptavidin.

After incubation, the wells are washed to remove unbound material.

A TMB substrate solution is added to the wells and color develops in proportion to the amount of target protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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