SpeedELISA Kits

Quantitative, Sandwich ELISAs for Detection of Soluble Factors in just Three Hours

The RayBio^® SpeedELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of target protein in biological sample such as serum, plasma, and cell culture supernatants.

Browse All SpeedELISA Kits

For a comparison & overview of all our ELISA systems, click here.

Features

Compressed workflow with only one wash step and three incubation steps
Only 3 hours total processing time
Strip plate format minimizes waste
Complete kit includes all necessary reagents
Highly sensitive and specific
Reproducible and reliable
Optimized for serum, plasma, urine and cell culture medium
Excellent for follow up/confirmation of antibody array data

Contents of Kit

Streptavidin-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Capture Antibody
HRP Conjugated Detection Antibody
TMB One-Step Substrate

Representative Data

Research Applications

Detection of quantitative protein levels in biological fluids
Validation of antibody array results
Validation of biomarker discovery studies

How It Works

RayBio^® SpeedELISAs employ a biotinylated capture and a HRP-conjugated detection antibody which immunocaptures the sample analyte in solution. The microplate in the kit is precoated with streptavidin.

The biotinylated capture antibody/protein/HRP-conjugated detection antibody mixture is pipetted into the wells and the biotinylated target protein present in a sample is bound to the wells by the immobilized streptavidin.

After incubation, the wells are washed to remove unbound material.

A TMB substrate solution is added to the wells and color develops in proportion to the amount of target protein bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.