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Stem Cell Factor, Human Recombinant
| Size | 10 µg, 1000 µg |
| Estimated Lead Time | 2-3 weeks |
| Species | Human |
| Accession Number | P21583 |
| Gene Id | 4254 |
| Protein Name / Synonyms | Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250. |
| Expressed Region | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Gly-Ile-Cys. |
| Expression System | E. coli |
| Purity | Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. |
| Format | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a concentrated (1mg/ml) solution in water containing 0.02% NaHCO3. |
| Activity | The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 500,000 IU/mg. |
| Reconstitution | It is recommended to reconstitute the lyophilized Stem Cell Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Research Area | Stem Cell Biology |
| Protein Content | Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) 3 solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
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Stem Cell Factor, Human Recombinant