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Trypsin, Sterile, Irradiated
Catalog #: 252-10155
Chromatographically purified, lyophilized, irradiated and tested for the absence of mycoplasma and extraneous virus according to 9 CFR113.53c. Each vial is filled to contain ≥100 mg.
Product Description
Specifications
| Size | 5x100 mg, 100 mg |
| Estimated Lead Time | 1-2 weeks |
| Accession Number | P00760 |
| Expression System | Bovine Pancreas |
| Molecular Weight (kDa) | 23.3 kDa (Theoretical) |
| Format | Lyophilized (powder) |
| Activity | ≥180 units per mg protein (at least 10,350 BAEE/3450 USP/NF units per mg protein) |
| Recommended Applications | Tissue dissociation, especially when combined with other enzymes such as collagenase, and elastase Cell harvesting by "trypsinization" Mitochondria isolation in vitro studies of proteins Removing monolayers of cells from plastic and glass Various hemagglu, Tissue dissociation, especially when combined with other enzymes such as collagenase, and elastase Cell harvesting by "trypsinization" Mitochondria isolation in vitro studies of proteins Removing monolayers of cells from plastic and glass Various hemagglutination procedures Sample preparation for flow cytometric DNA analysis Tryptic mapping Fingerprinting and sequencing work Environmental monitoring Reduction of cell density in tissue culture Subculturing cells Cleavage fusion proteins Generating glycopeptides from purified glycoproteins (White and White 1997) |
| Source | Bovine Pancreas |
| Shipping Type | Ambient temperature |
| Storage | 2-8°C |
Description
Other Information
Extinction Coefficient
Trypsinogen: 45,250 cm-1 M-1>
Isoelectric
Trypsinogen: pH 9.3 (Walsh and Neurath 1964) Trypsin: pH 10.5 (Cunningham 1954).
CATH
Class: Mainly Beta Architecture: Beta Barrel Topology: Thrombin, subunit H
CAS Registry Number
C.A.S.: 9002-07-7
Storage Conditions
Store at 2-8°C.