Mouse Anti-Mouse TSC2

Mouse Anti-Mouse TSC2 Antibody, 400 ul

$420.00  
102-10150
+

Antigen Information

Accession Number:
  • P49815
Gene ID:
  • 7249
Gene Symbols:
  • TSC2
Protein Name & Synonyms:
TSC2
Target Species:
  • Mouse

Antibody Specifications

Concentration:
0.5 mg/ml
Preparation:
This TSC2 monoclonal antibody is generated from mouse immunized with TSC2 recombinant protein.
Species Reactivity:
Mouse
Clonality:
Monoclonal

Introduction

Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.

Specificity

Other Names
TSC2; TSC4; Tuberin; Tuberous sclerosis 2 protein
NCBI Accession #
NP_000539.2;NP_001070651.1;NP_001107854.1
Other Accession #
NP_000539.2; NP_001070651.1

Format

Type
Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
200608
Recommended Dilutions
IF: 1:10-50; WB: 1:100
Fluorescent image of Hela cell stained with TSC2 Antibody(Cat# 102-10150/SG110509AA).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSC2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TSC2 immunoreactivity is localized to Microtubules significantly.
TSC2 Antibody (Cat# 102-10150) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TSC2 antibody detected the TSC2 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

No posts found

Write A Review

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.