25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

Ubiquitinated NEDD8 ELISA Kit

Measures ubiquitinated NEDD8 and total NEDD8 in human cell and tissue lysates, enabling precise assessment of NEDD8 ubiquitination by ELISA.

This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	4-8 weeks
Species	Human
Compatible Sample Types	Tissue Lysates, Cell Lysates
Accession Number	Q15843
Gene Id	4738
Gene Symbols	NEDD8
Protein Name / Synonyms	Ubiquitin-like protein NEDD8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8 (NEDD-8)
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Research Area	Post-Translational Modifications, Epigenetics
Modification	Ubiquitination
Shipping Type	Blue ice
Storage	-20°C

Introduction

The RayBio^® Ubiquitinated-NEDD8 and Total-NEDD8 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human ubiquitinated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan NEDD8 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and NEDD8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-ubiquitination antibody is used to detect ubiquitinated NEDD8 or biotinylated anti-NEDD8 antibody is used to detect total NEDD8 . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ubiquitinated NEDD8 or total NEDD8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Product Features

Quantify quickly and easily
Screen numerous different lysates without performing a Western Blot analysis
Minimal hands-on time, convenient, and non-radioactive material

Kit Components

Component	Size / Qty
Microplate Coated With Anti-Pan-NEDD8 Antibody	1 plate
Anti-Ubiquitination Detection Antibody	1 vial
Anti-NEDD8 Detection Antibody	1 vial
HRP-Conjugated Streptavidin	200 µL
Wash Buffer	25 mL
Assay Diluent	15 mL
Lysis Buffer	5 mL
TMB One-Step Substrate Reagent	12 mL
Stop Solution	8 mL

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Log-log graph paper or computer and software for ELISA data analysis
Absorbent paper
Tubes to prepare sample dilutions
Distilled or deionized water
Shaker

Assay Procedure Summary

Prepare all reagents and samples as instructed.
Add 100 µL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4°C with gentle shaking.
Add 100 µL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
Add 100 µL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
Add 50 µL Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Customization

This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com for more information.

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Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

Introduction

Product Features

Quantify quickly and easily
Screen numerous different lysates without performing a Western Blot analysis
Minimal hands-on time, convenient, and non-radioactive material

Kit Components

Component	Size / Qty
Microplate Coated With Anti-Pan-NEDD8 Antibody	1 plate
Anti-Ubiquitination Detection Antibody	1 vial
Anti-NEDD8 Detection Antibody	1 vial
HRP-Conjugated Streptavidin	200 µL
Wash Buffer	25 mL
Assay Diluent	15 mL
Lysis Buffer	5 mL
TMB One-Step Substrate Reagent	12 mL
Stop Solution	8 mL

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Log-log graph paper or computer and software for ELISA data analysis
Absorbent paper
Tubes to prepare sample dilutions
Distilled or deionized water
Shaker

Assay Procedure Summary

Prepare all reagents and samples as instructed.
Add 100 µL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4°C with gentle shaking.
Add 100 µL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
Add 100 µL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
Add 50 µL Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Customization

This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com for more information.

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25% off all ELISA kits

25% off all ELISA kits

Ubiquitinated NEDD8 ELISA Kit