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Ultrasensitive Biomarker Testing Service

1,000 Times More Sensitive Than ELISA

Single molecule array (Simoa™) testing is a powerful new technique that is orders of magnitude more sensitive than standard sandwich based immunoassay techniques. Traditional ELISA measurements are limited to pg/ml levels of detection. The RayBio Ultrasensitive Simoa Testing Services can achieve sensitivity as low as femtogram (fg/ml) levels, allowing the detection and quantification of biomarkers at concentrations previously difficult or impossible to measure.

Available Assays

RayBiotech is currently offering the below ultrasensitive assays for immediate testing:

TargetSensitivityGene SymbolAccession #ELISA Kit
Amyloid beta 1-40 LLOD: 0.522 pg/ml
LLOQ: 1.23 pg/ml
APP A4 AD1 P05067 ELISA Kit
BDNF LLOD: 0.2 pg/ml
LLOQ: 0.9 pg/ml
BDNF P23560 ELISA Kit
BLC (CXCL13) LLOD: 0.048 pg/ml
LLOQ: 0.07 pg/ml
CXCL13 BCA1 BLC SCYB13 O43927 ELISA Kit
CA-125 LLOD: 0.003 U/ml
LLOQ: 0.010 U/ml
MUC16 CA125 Q8WXI7 ELISA Kit
Cardiac Troponin I (cTnI) LLOD: 10 fg/ml
LLOQ: 40 fg/ml
TNNI3 TNNC1 P19429 ELISA Kit
Cathepsin S LLOD: 0.7 pg/ml
LLOQ: 1.95 pg/ml
CTSS P25774 ELISA Kit
CEA LLOD: 0.486 pg/ml
LLOQ: 2.33 pg/ml
CEACAM5 CEA P06731 ELISA Kit
C-Peptide LLOD: 0.013 pg/ml
LLOQ: 0.021 pg/ml
INS P01308 ELISA Kit
Endothelin-1 LLOD: 1.5 pg/ml
LLOQ: 3.2 pg/ml
EDN1 P05305 ELISA Kit
Eotaxin-1 (CCL11) LLOD: 0.1 pg/ml
LLOQ: 0.18 pg/ml
CCL11 SCYA11 P51671 ELISA Kit
G-CSF LLOD: 0.095 pg/ml
LLOQ: 0.095 pg/ml
CSF3 C17orf33 GCSF P09919 ELISA Kit
GDNF LLOD: 3 pg/ml
LLOQ: 9 pg/ml
GDNF P39905 ELISA Kit
GM-CSF LLOD: 0.0019 pg/ml
LLOQ: 0.0103 pg/ml
CSF2 GMCSF P04141 ELISA Kit
HGFR (c-MET) LLOD: 0.036 pg/ml
LLOQ: 0.224 pg/ml
MET P08581 ELISA Kit
HIV-p24 LLOD: 0.4 pg/ml
LLOQ: 3 pg/ml
p24 Q9WMW5 ELISA Kit
IFN-gamma LLOD: 0.03 pg/ml
LLOQ: 0.4 pg/ml
IFNG P01579 ELISA Kit
IL-1 alpha LLOD: 0.004 pg/ml
LLOQ: 0.01 pg/ml
IL1A IL1F1 P01583 ELISA Kit
IL-1 beta LLOD: 0.2 pg/ml
LLOQ: 0.8 pg/ml
IL1B IL1F2 P01584 ELISA Kit
IL-2 LLOD: 2 fg/ml
LLOQ: 10 fg/ml
IL2 P60568 ELISA Kit
IL-3 LLOD: 0.6 pg/ml
LLOQ: 2 pg/ml
IL3 P08700 ELISA Kit
IL-4 LLOD: 0.0046 pg/ml
LLOQ: 0.039 pg/ml
IL4 P05112 ELISA Kit
IL-5 LLOD: 0.012 pg/ml
LLOQ: 0.041 pg/ml
IL5 P05113 ELISA Kit
IL-6 LLOD: 0.05 pg/ml
LLOQ: 0.2 pg/ml
IL6 IFNB2 P05231 ELISA Kit
IL-7 LLOD: 0.02 pg/ml
LLOQ: 0.05 pg/ml
IL7 P13232 ELISA Kit
IL-8 LLOD: 0.05 pg/ml
LLOQ: 0.2 pg/ml
CXCL8 IL8 P10145 ELISA Kit
IL-10 LLOD: 0.01 pg/ml
LLOQ: 0.03 pg/ml
IL10 P22301 ELISA Kit
IL-12 p70 LLOD: 5 fg/ml
LLOQ: 10 fg/ml
IL12A NKSF1 P29459 ELISA Kit
IL-13 LLOD: 0.0055 pg/ml
LLOQ: 0.0257 pg/ml
IL13 NC30 P35225 ELISA Kit
IL-15 LLOD: 0.003 pg/ml
LLOQ: 0.00062 pg/ml
IL15 P40933 ELISA Kit
IL-16 LLOD: 0.23 pg/ml
LLOQ: 0.52 pg/ml
IL16 Q14005 ELISA Kit
IL-17A LLOD: 3 fg/ml
LLOQ: 15 fg/ml
IL17A CTLA8 IL17 Q16552 ELISA Kit
IL-22 LLOD: 1.13 pg/ml
LLOQ: 2.06 pg/ml
IL22 ILTIF ZCYTO18 UNQ3099/PRO10096 Q9GZX6 ELISA Kit
IL-23a LLOD: 0.132 pg/ml
LLOQ: 0.686 pg/ml
IL23A SGRF UNQ2498/PRO5798 Q9NPF7 ELISA Kit
IL-23 LLOD: 0.02 pg/ml
LLOQ: 0.086 pg/ml
IL23A; IL12B P29460, Q9NPF7  
IL-28A LLOD: 0.022 pg/ml
LLOQ: 0.069 pg/ml
IFNL2 IL28A ZCYTO20 Q8IZJ0 ELISA Kit
IL-33 LLOD: 0.023 pg/ml
LLOQ: 0.103 pg/ml
IL33 C9orf26 IL1F11 NFHEV O95760 ELISA Kit
IP-10 LLOD: 0.052 pg/ml
LLOQ: 0.177 pg/ml
CXCL10 INP10 SCYB10 P02778 ELISA Kit
Leptin LLOD: 2.46 pg/ml
LLOQ: 4.94 pg/ml
LEP OB OBS P41159 ELISA Kit
LIF LLOD: 0.015 pg/ml
LLOQ: 0.086 pg/ml
LIF HILDA P15018 ELISA Kit
MAPT (Tau) LLOD: 0.019 pg/ml
LLOQ: 0.061 pg/ml
MAPT MAPTL MTBT1 TAU P10636 ELISA Kit
MCP-1 LLOD: 1.6 pg/ml
LLOQ: 3.5 pg/ml
CCL2 MCP1 SCYA2 P13500 ELISA Kit
NAP-2 LLOD: 20 pg/ml
LLOQ: 60 pg/ml
PPBP CTAP3 CXCL7 SCYB7 TGB1 THBGB1 P02775 ELISA Kit
NRG1-alpha (HRG1-alpha) LLOD: 0.090 pg/ml
LLOQ: 0.3 pg/ml
NRG1 GGF HGL HRGA NDF SMDF Q02297 ELISA Kit
NRG1-beta 1 LLOD: 22.5 pg/ml
LLOQ: 55 pg/ml
NRG1 GGF HGL HRGA NDF SMDF Q02297 ELISA Kit
PLGF LLOD: 0.064 pg/ml
LLOQ: 0.3 pg/ml
PGF PGFL PLGF P49763 ELISA Kit
PSA-free LLOD: 0.015 pg/ml
LLOQ: 0.024 pg/ml
KLK3 APS P07288 ELISA Kit
TGF-alpha LLOD: 0.04 pg/ml
LLOQ: 0.25 pg/ml
TGFA P01135 ELISA Kit
TGF-beta 1 LLOD: 0.137 pg/ml
LLOQ: 0.514 pg/ml
TGFB1 TGFB P01137 ELISA Kit
TNF-alpha LLOD: 10 fg/ml
LLOQ: 40 fg/ml
TNF TNFA TNFSF2 P01375 ELISA Kit
TNF-beta LLOD: 0.052 pg/ml
LLOQ: 0.15 pg/ml
LTA TNFB TNFSF1 P01374 ELISA Kit
TRAIL LLOD: 0.0083 pg/ml
LLOQ: 0.0177 pg/ml
TNFSF10 APO2L TRAIL P50591 ELISA Kit
UCH-L1 (PGP9.5) LLOD: 1.05 pg/ml
LLOQ: 3.43 pg/ml
UCHL1 P09936 ELISA Kit
VEGF-A LLOD: 0.045 pg/ml
LLOQ: 0.103 pg/ml
VEGFA VEGF P15692 ELISA Kit
TargetSensitivityGene SymbolAccession #ELISA Kit
GM-CSF LLOD: 0.04 pg/ml
LLOQ: 0.09 pg/ml
Csf2rb Aic2b Csf2rb1 Il3rb1 P26955 ELISA Kit
IL-1 beta LLOD: 0.6 pg/ml
LLOQ: 0.3 pg/ml
Il1b P10749 ELISA Kit
IL-10 LLOD: 0.05 pg/ml
LLOQ: 0.15 pg/ml
Il10, Il-10 P18893 ELISA Kit
IL-17A LLOD: 0.1 pg/ml
LLOQ: 0.3 pg/ml
Il17a Ctla8 Il17 Q62386 ELISA Kit
IL-17F LLOD: 0.15 pg/ml
LLOQ: 0.5 pg/ml
Il17f Q7TNI7 ELISA Kit
IL-22 LLOD: 0.1 pg/ml
LLOQ: 0.25 pg/ml
Il22 Il22a Iltif Iltifa Q9JJY9 ELISA Kit
IL-23 LLOD: 0.05 pg/ml
LLOQ: 0.15 pg/ml
Il23a; Il12b Q9EQ14, P43432  
IL-6 LLOD: 0.04 pg/ml
LLOQ: 0.15 pg/ml
Il6 Il-6 P08505 ELISA Kit
TNF-alpha LLOD: 0.2 pg/ml
LLOQ: 1.5 pg/ml
Tnf Tnfa Tnfsf2 P06804 ELISA Kit
TargetSensitivityGene SymbolAccession #ELISA Kit
IL-1 beta LLOD: 2 pg/ml
LLOQ: 5 pg/ml
Il1b Q63264 ELISA Kit

View the Complete List of Biomarkers Available

To receive a quote*, fill out the sample service form linked below and email it to orders@raybiotech.com.

*10 sample minimum

Sample Service FormSample Shipping Instructions

How It Works

Simoa is based upon the isolation of individual immunocomplexes on paramagnetic beads using standard ELISA reagents. The main difference between Simoa and conventional immunoassays lies in the ability to trap single molecules in femtoliter-sized wells, allowing for a “digital” readout of each individual bead to determine if it is bound to the target analyte or not.

Simoa Sensitivity Comparison

Representative Data

Ultrasensitive IL-6 assay

IL-6 Multiday Testing


  Day 1 Day 2 Day 3 Mean CV
Serum 1 2.8845 2.8332 2.7788 2.8322 1.9%
Serum 2 1.8079 1.8785 1.8792 1.8552 2.2%
Serum 3 2.2035 2.1724 2.2078 2.1946 0.9%

References

Free Basic Biostatistics Report Free Basic Biostatistics Report