Auto-Western Service

Western Blotting Just Got Easier

Leave the burden of validating antibodies and optimizing conditions to us. RayBiotech's Auto-Western Service marries our state-of-the-art capillary immunoprobing system with our vast pre-validated antibody library. Our high throughput system can achieve ng to pg sensitivity with only 5 µL of starting material. Just send us your samples, select your desired antibody from our pre-validated list, and we'll do the rest. You can even send us your own antibody.

Service Features

  • Low sample volume required (5 µl)
  • As low as 0.2 µg/µl protein
  • Ng to pg sensitivity
  • Full analysis report included (see example below)
  • Standard curve may be included for full quantification (additional charges apply)
Western blot image

How It Works

Auto-Western: How It Works

RayBiotech's auto-western service uses a highly accurate and precise capillary immunoassay method. Samples and reagents are loaded into an assay plate and placed in our western machine. As little as 40 nL of sample is loaded into the capillary automatically and separated by size as they migrate through a stacking and separation matrix. The separated proteins are then immobilized to the capillary wall via a proprietary, photoactivated capture chemistry. Target proteins are identified using a primary antibody and immunoprobed using an HRP-conjugated secondary antibody and chemiluminescent substrate. The resulting chemiluminescent signal is detected and quantitated.

Get a Quote Today!

For as little as $35 per sample*, you will receive a data report with signal peak diagrams and a digitized virtual gel image. To obtain a service quotation, simply fill out a quote request form linked below or email our ordering department ([email protected]). They will email you back a formal quote as soon as possible. Above pricing for North America customers only.

*When testing with an antibody from RayBiotech's Western Library

Ready To Ship Your Samples?

Fill out the array service form linked below and include it with your shipment to RayBiotech. We will notify you once we receive the samples and start the service.

Representative Data

Results are presented in electropherogram format (A) with each peak displayed numerically (B). Electropherogram peaks are then digitally rendered as a "virtual blot" (C).


Western Blot Electropherogram


Western Blot Electropherogram


Virtual Western Blot

Quality Control Testing Data

RayBiotech's Auto-Western platform exhibits linear signal response down to the picogram (pg) range.

Electropherogram QC Western Line Graph QC