The E.Coli derived Recombinant Zika Envelope protein (250-410 a.a., Strain: ZikaSPH2015) having an Mw of 19kDa is designed with minimal level cross-reaction to other Flavivirus such as dengue and West Nile viruses. The Zika Envelope protein is fused to a 6xHis tag at C-terminus and purified by proprietary chromatographic technique.