Glucagon-like peptide-1 (GLP-1) is a highly conserved amino-acid-long peptide hormone among mammals and mainly secreted from the intestinal L-cells and the CNS. Functionally, GLP-1 plays an important role in energy homeostasis and nutrient absorption. The initial product GLP-1 (1–37) is susceptible to amidation and proteolytic cleavage, which gives rise to the two truncated or equipotent biologically active forms, GLP-1 (7–36) and GLP-1 (7–37) amide. Then, the active forms of GLP-1 are extremely susceptible to the catalytic activity of the proteolytic enzyme dipeptidyl peptidase-4 (DPP-4), resulting in the abundant inactivated GLP-1 (9–36) or GLP-1 (9–37) amide constituting 60–80% of total GLP-1 in circulation. This kit could specifically measure the active (7-36 and 7-37 amide) GLP-1, while not the original (1-37 amide) and inactive (9-36 and 9-37 amide) forms of GLP-1.

The RayBio^® active GLP-1 Enzyme Immunoassay (EIA) Kit (EIA-AGLP1) is a competitive enzyme immunoassay developed for rapid detection and quantitation of active GLP-1 in serum and plasma samples collected from various species including humans, porcine, feline, canine, rabbit, equine and sheep, but not rats or mice. The quantity of active GLP-1 is determined by a known standard GLP-1 curve. The active GLP-1 kit has a high sensitivity of approximately 0.071 ng/ml. The performance was also verified to exhibit good characteristics in terms of linearity, stability, precision, and spiking & recovery. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

• Pre-Coated 96-well Strip Microplate
• Assay Diluent
• Lyophilized Standard
• Positive Control
• Capture Antibody
• HRP-conjugated secondary antibody
• Wash Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1-liter graduated cylinders
• Distilled or deionized water
• Tubes to prepare standard or sample dilutions
• Orbital shaker
• Software which can perform four-parameter logistic regression models

1. Prepare all reagents, samples and standards as instructed.
2. Add 50 µL standards or samples to appropriate well. Incubate for 10 minutes at room temperature or 37°C.
3. Add 50 µL Capture Antibody to appropriate well. Incubate 1 hour at room temperature or 37°C. And wash 5 times.
4. Add 100 µL prepared HRP-Secondary antibody solution. Incubate 1 hour at room temperature or 37ºC. And wash 5 times.
5. Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 2-10 minutes at room temperature or 37°C.
6. Add 50 µL Stop Solution to each well. Read at 450 nm immediately.

The entire kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. For extended storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles.