15-PGDH antibody [C2C3], C-Terminus, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 3248 |
| Protein Name / Synonyms | 15-PGDH, C-term |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Neuroscience |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
15-PGDH antibody, PGDH antibody, PGDH1 antibody, SDR36C1 antibody, HPGD antibody, prostaglandin dehydrogenase 1 antibody, NAD+-dependent 15-hydroxyprostaglandin dehydrogenase antibody, 15-hydroxyprostaglandin dehydrogenase [NAD+] antibody, "short chain dehydrogenase/reductase family 36C, member 1 antibody", hydroxyprostaglandin dehydrogenase 15-(NAD) antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
29 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 205 and 266 of 15-PGDH (Uniprot ID# P15428)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 15-PGDH(107-10465) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using 15-PGDH(107-10465) antibody at 1:500 dilution.
Sample (30µg whole cell lysate)
A:Raji
12% SDS PAGE
107-10465 diluted at 1:1000
A:Raji
12% SDS PAGE
107-10465 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.