Rabbit Anti-Human ABI1 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q8IZP0 |
| Gene Id | 10006 |
| Gene Symbols | ABI1 |
| Protein Name / Synonyms | ABI1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This ABI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the N-terminal region of human ABI1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
ABI1 has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, this protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
ABI1; SSH3BP1; Abl interactor 1; Abelson interactor 1; Abl-binding protein 4; Eps8 SH3 domain-binding protein; Nap1-binding protein; Spectrin SH3 domain-binding protein 1; e3B1
NCBI Accession #
NP_001012768.1;NP_001012769.1;NP_001012770.1;NP_001171587.1;NP_001171590.1;NP_001171591.1;NP_001171592.1;NP_001171593.1;NP_001171594.1;NP_001171595.1;NP_001171596.1;NP_005461.2
Other Accession #
P62484; Q9NYB9; Q9QZM5; Q8CBW3
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
55081
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50
 | Western blot analysis of ABI1 Antibody (N-term) (Cat# 102-17906) in MCF-7, CEM, Jurkat cell line lysates (35ug/lane). ABI1 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human brain tissue reacted with ABI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of SK-Br-3 cells using ABI1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Immunofluorescence analysis of ABI1 Antibody (N-term) with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.