AKR1C1 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 1645 |
| Protein Name / Synonyms | AKR1C1 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunohistochemistry, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
2-ALPHA-HSD antibody, 20-ALPHA-HSD antibody, C9 antibody, DD1 antibody, DDH antibody, DDH1 antibody, H-37 antibody, HAKRC antibody, MBAB antibody, MGC8954 antibody, AKR1C1 antibody, 20-alpha-hydroxysteroid dehydrogenase antibody, dihydrodiol dehydrogenase 1/2 antibody, trans-1,2-dihydrobenzene-1,2-diol dehydrogenase antibody, high-affinity hepatic bile acid-binding protein antibody, dihydrodiol dehydrogenase isoform DD1 antibody, indanol dehydrogenase antibody, aldo-keto reductase family 1 member C1 antibody, aldo-keto reductase C antibody, type II 3-alpha-hydroxysteroid dehydrogenase antibody, chlordecone reductase homolog HAKRC antibody, hepatic dihydrodiol dehydrogenase antibody, 20 alpha-hydroxysteroid dehydrogenase antibody, dihydrodiol dehydrogenase 1 antibody, aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1, 20-alpha (3-alpha)-hydroxysteroid dehydrogenase) antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
37 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 22 and 248 of AKR1C1 (Uniprot ID# Q04828)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunohistochemistry,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed A431, using AKR1C1(107-10539) antibody at 1:200 dilution.
AKR1C1 antibody detects AKR1C1 protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
10 % SDS-PAGE
AKR1C1 antibody (107-10539) dilution: 1:1000
A. 50 µg mouse liver lysate/extract
10 % SDS-PAGE
AKR1C1 antibody (107-10539) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft , using AKR1C1(107-10539) antibody at 1:500 dilution.
Sample(30 ug whole cell lysate)
A:Raji
10% SDS PAGE
107-10539 diluted at 1:1000
A:Raji
10% SDS PAGE
107-10539 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.