Product Description

Specifications

Size	20 µg, 100 µg, 200 µg, 500 µg
Estimated Lead Time	1-2 business days
Species	Human
Specificity	This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat ALPP has not been tested yet.
Accession Number	P05187
Gene Id	250
Gene Symbols	ALPP PLAP
Clonality	Monoclonal
Immunogen	Immunogen was recombinant protein. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
Recommended Applications	ELISA (recommended work dilution= 1:40, 000), Western Blotting (recommended work dilution= 1:1, 000-5, 000),
Antigen Catalog Number	230-00257
Reconstitution	Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
Shipping Type	Ambient temperature
Storage	-20°C

Description

Alkaline Phosphatase(ALPP), placental type is a membrane bound glycosylated dimeric enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form.Itis a tumor marker, especially in seminoma and ovarian cancer.

Typical Data

Cell Lysate: SAOS2 0.2 mg/ml | Secondary Ab: Mouse | Exposure: 5 sec | Expected MW (kDa): 55

Cell Lysate: Hela 0.2 mg/ml | Secondary Ab: Mouse | Exposure: 5 sec | Expected MW (kDa): 55

Cell Lysate: HEPG2 0.2 mg/ml | Secondary Ab: Mouse | Exposure: 5 sec | Expected MW (kDa): 55

Cell Lysate: 143B 0.2 mg/ml | Secondary Ab: Mouse | Exposure: 5 sec | Expected MW (kDa): 55

Storage/Stability

Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -97°C. After reconstitution, it can be aliquoted and storedetory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been st