alpha SNAP antibody [N2C3], 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 8775 |
| Protein Name / Synonyms | alpha SNAP |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
SNAPA antibody, NAPA antibody, alpha-SNAP antibody, alpha-soluble NSF attachment protein antibody, "N-ethylmaleimide-sensitive factor attachment protein, alpha antibody"
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
33 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 78 and 295 of alpha SNAP (Uniprot ID# P54920)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, ICC/IF: 1:100-1:1000, IHC-P: 1-100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed A431, using alpha SNAP(107-10633) antibody at 1:200 dilution.
alpha SNAP antibody [N2C3] detects alpha SNAP protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
alpha SNAP antibody [N2C3] (107-10633) dilution: 1:500.
Sample: Paraffin-embedded mouse lung.
alpha SNAP antibody [N2C3] (107-10633) dilution: 1:500.
Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
107-10633 diluted at 1:10000
A: mouse brain
12% SDS PAGE
107-10633 diluted at 1:10000
Sample(30 ug whole cell lysate)
A:Hep G2
10% SDS PAGE
107-10633 diluted at 1:1000
A:Hep G2
10% SDS PAGE
107-10633 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.