Amphiphysin antibody [N1N2], N-Terminus, 0.9 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 273 |
| Protein Name / Synonyms | Amphiphysin, N-term |
| Concentration (lot specific) | 0.9 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
AMPH1 antibody, AMPH antibody, amphiphysin antibody, amphiphysin I antibody, amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
76 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 194 of Amphiphysin (Uniprot ID# P49418)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Amphiphysin antibody [N1N2], N-term (107-10373) dilution: 1:500.
Sample: Paraffin-embedded U87 xenograft.
Amphiphysin antibody [N1N2], N-term (107-10373) dilution: 1:500.
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Amphiphysin antibody [N1N2], N-term (107-10373) dilution: 1:500.
Sample: Paraffin-embedded mouse brain.
Amphiphysin antibody [N1N2], N-term (107-10373) dilution: 1:500.
Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
Amphiphysin antibody [N1N2], N-term (107-10373) dilution: 1:500.
Sample: Paraffin-embedded RT2 xenograft.
Amphiphysin antibody [N1N2], N-term (107-10373) dilution: 1:500.
Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis.
A. 50 µg mouse brain lysate/extract
7.5 % SDS-PAGE
Amphiphysin antibody [N1N2], N-term (107-10373) dilution: 1:1000
A. 50 µg mouse brain lysate/extract
7.5 % SDS-PAGE
Amphiphysin antibody [N1N2], N-term (107-10373) dilution: 1:1000
Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
5 % SDS-PAGE
Amphiphysin antibody [N1N2], N-term (107-10373) dilution: 1:500
A. 50 µg rat brain lysate/extract
5 % SDS-PAGE
Amphiphysin antibody [N1N2], N-term (107-10373) dilution: 1:500
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Amphiphysin(107-10373) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.