Rabbit Anti-Human AMPK alpha2 (PRKAA2) (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P54646 |
| Gene Id | 5563 |
| Gene Symbols | PRKAA2 |
| Protein Name / Synonyms | AMPK alpha2 (PRKAA2) (C-term) |
| Clonality | Polyclonal |
| Preparation | This AMPK alpha2 (PRKAA2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 453-483 amino acids from the C-terminal region of human AMPK alpha2 (PRKAA2). |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | AMPK Signaling, Autophagy, Energy Metabolism |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
PRKAA2; AMPK; AMPK2; 5'-AMP-activated protein kinase catalytic subunit alpha-2; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase
NCBI Accession #
NP_006243.2
Other Accession #
Q09137; Q8BRK8
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
62320
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50
 | Western blot analysis of PRKAA2 (arrow) using rabbit polyclonal PRKAA2 Antibody (C-term) (RB11657). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA2 gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PRKAA2 antibody (C-term)(Cat# 102-17331), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.