[KO Validated] APEX1 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P27695 |
| Gene Id | 328 |
| Gene Symbols | APEX1 |
| Protein Name / Synonyms | APEX1, APE, APE1, APEN, APEX, APX, HAP1, REF1, DNA-(apurinic or apyrimidinic site) lyase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
Format
Calculated MW
35kDa
Observed MW
39kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1 (NP_542380.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using APEX1 antibody (144-61347).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.![Western blot - [KO Validated] APEX1 Polyclonal Antibody (144-61347)](https://cdn.sanity.io/images/g51potcz/production/6cf1abd6f9c1706f4b5f6c99bd622b1c8c06d8bf-237x350.jpg)
Western blot analysis of extracts from normal (control) and APEX1 knockout (KO) 293T cells, using APEX1 antibody (144-61347) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] APEX1 Polyclonal Antibody (144-61347)](https://cdn.sanity.io/images/g51potcz/production/7edbd1807ed53c1f1d849d674108e9c5c3e5a5f3-366x550.jpg)
Immunofluorescence analysis of HeLa cells using APEX1 antibody (144-61347). Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] APEX1 Polyclonal Antibody (144-61347)](https://cdn.sanity.io/images/g51potcz/production/e549ce34f1617e9b60560a38f21fce67aec19d7d-468x350.jpg)
Immunofluorescence analysis of A549 cells using APEX1 antibody (144-61347).![Immunofluorescence - [KO Validated] APEX1 Polyclonal Antibody (144-61347)](https://cdn.sanity.io/images/g51potcz/production/0b438689df9d371fb6b4bfd845c7bee388cd1ab4-330x350.jpg)
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug APEX1 antibody (144-61347). Western blot was performed from the immunoprecipitate using APEX1 antibody (144-61347) at a dilition of 1:1000.![Immunoprecipitation - [KO Validated] APEX1 Polyclonal Antibody (144-61347)](https://www.raybiotech.com/files/images/Antibodies/144/144-61347/5.jpg)
Expiration:
12 months from the date of shipment when stored properly.
